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	Provedor de dados BJM (2) Autor Karimi,Vahid (2) Salehi,Taghi Zahraei (2) Derakhshandeh,Abdollah (1) Fasaei,Bahar Nayeri (1) Jahromi,Omid Ali Nekoui (1) Khazaeli,Fatemeh Arab (1) Madani,Seyed Ahmad (1) Tabatabaei,Saeid (1) Mais... Palavra-chave Avian pathogenic Escherichia coli (1) Chickens (1) Escherichia coli (1) Iran (1) Molecular differentiation (1) RAPD-PCR (1) Vaccine (1) ΔaroA mutant (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Assessment of immunity against avian colibacillosis induced by an aroA mutant containing increased serum survival gene in broilers BJM Salehi,Taghi Zahraei; Tabatabaei,Saeid; Karimi,Vahid; Fasaei,Bahar Nayeri; Derakhshandeh,Abdollah; Jahromi,Omid Ali Nekoui. Colibacillosis is an important disease in the poultry industry which causes serious economic damages. As it is suggested that vaccination is one of the means to control colibacillosis, we tried to investigate the vaccine potential of a ΔaroA derivative of an O78:K80 avian pathogenic Escherichia coli containing increased serum survival gene. 490 chicks were selected as follows: For assessment of virulence of ΔaroA mutant, 30 chicks were divided into three groups and injected with 0.5ml of PBS or bacterial suspension containing either10(7)colony forming units (CFU) of mutant or parent strains via subcutaneous route. Macroscopic lesions and mortality rate were recorded in different groups during the week after challenge. For assessment of safety and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickens; Avian pathogenic Escherichia coli; ΔaroA mutant; Vaccine. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100043 Molecular genetic differentiation of avian Escherichia coli by RAPD-PCR BJM Salehi,Taghi Zahraei; Madani,Seyed Ahmad; Karimi,Vahid; Khazaeli,Fatemeh Arab. Escherichia coli is one of the most important bacterial avian pathogens and a common inhabitant of the gastrointestinal tract of animals. Most pathogenic E. coli can not be differentiated biochemically or by classic microbiologic methods. Molecular typing methods, particularly PCR, facilitated epidemiological and ecological studies of bacteria. Here we describe the application of a random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) for molecular genetic differentiation of E. coli isolates in Iran. In this study 58 E. coli isolates including 4 standard strains, 3 food originated isolates, 33 avian isolates, 8 isolates form diarrheic calves and 10 isolates from unweaned diarrheic lambs were analyzed by RAPD-PCR using primer 1247(5/-AAG... Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; RAPD-PCR; Molecular differentiation; Iran. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300015 Registros recuperados: 2 Primeira ... 1 ... Última

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